GuideX

crispr/cas13 software that identifies conserved regions, designs cas13 gRNAs, performs off-target analysis, and completes gRNA optimization.

Requirements

export NCBI_API_KEY_2025="your_ncbi_key"
python3 test_guidex.py

Name		Name	Last commit message	Last commit date
Latest commit History 376 Commits
config		config
graphs		graphs
guidex		guidex
LICENSE		LICENSE
README.md		README.md
actual_test_guidex.py		actual_test_guidex.py
alignment_engine.py		alignment_engine.py
another_test_guidex.py		another_test_guidex.py
conservation.py		conservation.py
genome_fetcher.py		genome_fetcher.py
grna_designer.py		grna_designer.py
ncbi_test.py		ncbi_test.py
off_target.py		off_target.py
old_alignment_engine.py		old_alignment_engine.py
old_conservation.py		old_conservation.py
old_off_target.py		old_off_target.py
old_test_guidex.py		old_test_guidex.py
optimizer.py		optimizer.py
setup.py		setup.py
setup_cbsv_genomes.py		setup_cbsv_genomes.py
test_api.py		test_api.py
test_guidex.py		test_guidex.py
test_guidex_no_local_genomes.py		test_guidex_no_local_genomes.py
visualization.py		visualization.py