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@Coppini Coppini commented Feb 20, 2025

fastp already sets --trim_poly_g for two-color-systems (NEXTSEQ500, NEXTSEQ 550/550DX, NOVASEQ), but does not do the same for iSeq, which is a one color system.

Users of iSeq face the same poly-G issues as those of the other platforms mentioned, so it would make sense to also trim poly-Gs by default.

@semenko
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semenko commented Apr 15, 2025

This might be missing a few (like VL and VH) -- I started a PR a while back in #508 but I don't think it was ever disscused more.

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2 participants