Project 1_2: Modeling doxycycline induced GFP expression systems without feedback

Requirement

1. pCMV is a constitutive promoter
2. Black triangle is a tetR-dimer binding site (tetO), there are two of them near TATA box
3. NLS is nuclear localization sequence, facilitate the translocation of tetR to nucleus
4. In the absence of Doxcycline, tetR-dimer preferably binds to tetO, block the RNA polymerase
5. In the presence of Doxcycline, tetR-dimer preferably disassociate from tetO, remove the interference to RNA polymerase Please

• write step-by-step reactions;
• write ODE equations from reactions;
• simplified the model with quasi-steady state assumption, please justified the assumptions
• play with the parameters to minic data from fig C

Analysis

Reaction formula --> ODE formal Varioution of Cell

• pCMC --(k0)--> tetR ~~~~~~~~~~~~~~~~~[1]
• tetR + tetR --(k1)--> tetR2(Dimer)~~~~~[2]
• tetR2+ Dox --(k2)--> tetR2*Dox ~~~~~~~[3]

Hypothesis:

• Dox is uniform distribution in culture.

Result Analysis