@@ -10,7 +10,9 @@ configfile: "sd_analysis.json"
1010if "asm" not in config and "assembly" in config :
1111 config ["asm" ] = config ["assembly" ]
1212
13- asmFai = config ["asm" ] + ".fai"
13+ assembly = "assembly.orig.fasta"
14+ asmFai = "assembly.orig.fasta.fai"
15+ #asmFai=config["asm"] + ".fai"
1416asmFaiFile = open (asmFai )
1517contigs = [l .split ()[0 ] for l in asmFaiFile ]
1618strIdx = [str (i ) for i in range (0 ,len (contigs ))]
@@ -27,7 +29,7 @@ rule all:
2729
2830rule SplitContig :
2931 input :
30- asm = config [ "asm" ]
32+ asm = assembly
3133 output :
3234 contig = "split/to_mask.{index}.fasta" ,
3335 params :
@@ -78,26 +80,31 @@ rule SpecialMaskContig:
7880 tmpdir = tempDir ,
7981 sd = SD
8082 shell :"""
81- mkdir -p t2t
82- TEMP="$TMPDIR/$$_$RANDOM/"
83- mkdir -p $TEMP
84- #
85- # Copy input file to temp dir that should have fast IO
86- #
87- {params.sd}/hardmask {input.mask} $TEMP/to_mask.{wildcards.index}.fasta && \
88- pushd $TEMP && \
89- RepeatMasker -nolow -libdir /home1/mchaisso/miniconda3/share/RepeatMasker/Libraries/Appended -species human -pa 8 -s -xsmall \" to_mask.{wildcards.index}.fasta\" && \
90- popd && \
83+ if [ {params.repeatLibrary} != "na" ]
84+ then
85+ mkdir -p t2t
86+ TEMP="$TMPDIR/$$_$RANDOM/"
87+ mkdir -p $TEMP
88+ #
89+ # Copy input file to temp dir that should have fast IO
90+ #
91+ {params.sd}/hardmask {input.mask} $TEMP/to_mask.{wildcards.index}.fasta && \
92+ pushd $TEMP && \
93+ RepeatMasker -nolow -libdir /home1/mchaisso/miniconda3/share/RepeatMasker/Libraries/Appended -species human -pa 8 -s -xsmall \" to_mask.{wildcards.index}.fasta\" && \
94+ popd && \
9195
92- if [ ! -e $TEMP/to_mask.\" {wildcards.index}\" .fasta.masked ]; then
93- ls -l masked/to_mask.\" {wildcards.index}\" .fasta.masked
94- cp masked/to_mask.\" {wildcards.index}\" .fasta.masked t2t/to_mask.\" {wildcards.index}\" .fasta.masked
95- head -3 masked/to_mask.\" {wildcards.index}\" .fasta.out > t2t/to_mask.\" {wildcards.index}\" .fasta.out
96+ if [ ! -e $TEMP/to_mask.\" {wildcards.index}\" .fasta.masked ]; then
97+ ls -l masked/to_mask.\" {wildcards.index}\" .fasta.masked
98+ cp masked/to_mask.\" {wildcards.index}\" .fasta.masked t2t/to_mask.\" {wildcards.index}\" .fasta.masked
99+ head -3 masked/to_mask.\" {wildcards.index}\" .fasta.out > t2t/to_mask.\" {wildcards.index}\" .fasta.out
100+ else
101+ cp $TEMP/to_mask.\" {wildcards.index}\" .fasta.* t2t/ || true
102+ fi
103+ rm -rf $TEMP
96104else
97- cp $TEMP/to_mask.\" {wildcards.index}\" .fasta.* t2t/ || true
105+ echo "*** rule SpecialMaskContig made no change (skipped) ***" > t2t/to_mask.\" {wildcards.index}\" .fasta.out
106+ cp {input.mask} t2t/to_mask.\" {wildcards.index}\" .fasta.masked >> t2t/to_mask.\" {wildcards.index}\" .fasta.out || true
98107fi
99- rm -rf $TEMP
100-
101108"""
102109
103110rule MergeMaskerRuns :
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