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src/qp_klp/PacBioMetagenomicWorkflow.py

Lines changed: 1 addition & 1 deletion
Original file line numberDiff line numberDiff line change
@@ -40,7 +40,7 @@ def __init__(self, **kwargs):
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samples = [
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{'barcode': sample['barcode_id'],
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'sample_name': sample['Sample_Name'],
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'sample_name': sample['Sample_ID'],
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'project_name': sample['Sample_Project'],
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'lane': sample['Lane']}
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for sample in self.pipeline.sample_sheet.samples]

src/qp_klp/scripts/pacbio_commands.py

Lines changed: 2 additions & 1 deletion
Original file line numberDiff line numberDiff line change
@@ -36,8 +36,9 @@ def generate_bam2fastq_commands(sample_list, run_folder, outdir, threads):
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missing_files.append(bc)
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continue
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od = f'{outdir}/{pn}'
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makedirs(od, exist_ok=True)
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fn = f'{od}/{sn}_L00{lane}_R1_001'
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fn = f'{od}/{sn}_S000_L00{lane}_R1_001'
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cmd = (f'bam2fastq -j {threads} -o {fn} -c 9 '
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f'{files[bc]}; '
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f'fqtools count {fn}.fastq.gz > '

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