diff --git a/samplesheets/plant_ts_samplesheet.tsv b/samplesheets/plant_ts_samplesheet.tsv
new file mode 100644
index 000000000..906254d97
--- /dev/null
+++ b/samplesheets/plant_ts_samplesheet.tsv
@@ -0,0 +1,4 @@
+sampleID	forwardReads	reverseReads	run
+sample_1	https://github.com/nf-core/test-datasets/raw/ampliseq/testdata/plant_ts_1_R1.fastq.gz	https://github.com/nf-core/test-datasets/raw/ampliseq/testdata/plant_ts_1_R2.fastq.gz	1
+sample_2	https://github.com/nf-core/test-datasets/raw/ampliseq/testdata/plant_ts_2_R1.fastq.gz	https://github.com/nf-core/test-datasets/raw/ampliseq/testdata/plant_ts_2_R2.fastq.gz	2
+
diff --git a/testdata/plant_ts_1_R1.fastq.gz b/testdata/plant_ts_1_R1.fastq.gz
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